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Th. C. Singh ¹, S. Biswas ², S. Dasgupta ², A. Mitra ³, A. K. Panda ⁴, R. K. Nath ²

Affiliations
1 Department of Chemistry, D. D. M. College, Khowai, Tripura-799 202, IN
2 Department of Chemistry, Tripura University, Suryamaninagar, Tripura-799 130, IN
3 Department of Chemistry, N. S. Mahavidyalaya, Udaipur, Tripura-799 120, IN
4 Department of Chemistry, University of North Bengal, P.O. North Bengal University, District:Darjeeling, West Bengal-734 013, IN

Abstract

Spectral studies on the interaction of capsular polysaccharide (SPS) isolated from Klebsiella serotype K34, with oppositely charged dyes and surfactants have been reported. The SPS was acidic in nature and induced strong metachromacy (∼110 nm blue shift) in the cationic dye pinacyanol chloride (PCYN) which was due to "card-pack stacking" of individual dye monomers on the surface of the polyanions. Reversal of metachromacy offered a qualitative measurement of stability and nature of binding associated with PCYN-SPS complex. Thermodynamic parameters of dye-polymer complex were evaluated. SPS-cationic dye acridine orange (AO) interaction in aqueous solution have been investigated fluorimetrically. Dye incorporation technique was employed to study cationic surfactant-polymer interactions. Interactions between the polyelectrolyte and oppositely charged surfactants lead to the formation of induced premicelles at surfactant concentrations lower than the CMC of the surfactants and the corresponding binding constant was evaluated. Such studies revealed that the surfactant is not exclusively bound electrostatically, but also through hydrophobic interactions.

Keywords

Dye, Surfactant, SPS, Klebsiella, Binding Constant.

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Th. C. Singh ¹, R. K. Nath ¹, K. Manna ², A. K. Panda ³

Affiliations
1 Department of Chemistry, Tripura University, Suryamaninagar, Tripura-799 130, IN
2 Deparatment of Chemistry, Gyan Jyoti College, Siliguri, West Bengal, IN
3 Department of Chemistry, University of North Bengal, P.O. North Bengal University, D Darjeeling, West Bengal-734 430, IN

Abstract

Capsular polysaccharide (SPS) is an integral component of gram negative bacteria, Klebsiella. The interaction of SPS isolated from Klebsiella K40 serotype with oppositely charged pure surfactants and cationic-nonionic mixed surfactants were investigated by dynamic light scattering and viscometric technique. Variation in the hydrodynamic radius and zeta potential were studied by dynamic light scattering method. Nature of the interaction is governed by concentration of the surfactants, charge density, surfactant head group and the CMC of the surfactants. Micellar effects of the pure and mixed surfactants on binding with the SPS are presented in this paper.

Keywords

Capsular Polysaccharide, Dynamic Light Scattering, Viscometry, CMC.

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Shankar Sarbajna ¹, N. Choudhury ¹, J. Mitra ¹, G. Mukherjee ¹, G. C. Sarkar ¹, R. K. Nath ¹, M. K. Chakraborty ¹

Affiliations
1 Department of Obstetrics & Gynaecology, Institute of Post Graduate Medical Education and Research, Calcutta, IN

Abstract

No Abstract.

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R. K. Nath ¹, A. Chakrabarti ¹, A. K. Chakraborty ¹

Affiliations
1 Department of Chemistry, Calcutta University Post Graduate Centre, Agartala, Tripura-799004, IN

Abstract

Interaction of cationic dye pinacyanol chloride with bacterial cell surface capsular polysaccharide isolated from Klebsiella K40 has been spectrophotometrically Investigated. The capsular polysaccharide of Klebsiella K40 is a heteroglycan which consists of pentasaccharide repeating units comprising one residue each of unsubstituted mannose, 4-substituted glucuronic acid, 3-substituted galactose, 2, 4-disubstituted rhamnose, and 2-substituted rhamnose. It Induces a metachromatic blue shift in the visible spectrum of the pinacyanol chloride from 600 nm to 500 nm. The metachromatic spectral changes have been studied during interaction of the dye cation with the polyanion at different polymer/dye molar ratios. A stochiometry of 11 for the polymer/dye has been found to exist in the polyanion-dye compound. The complete reversal of polymer-induced metachromasy has also been observed by the addition of ethanol and urea.

Keywords

Pinacyanol Chloride, Poly Saccharide, Metachromasy.

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R. K. Nath ¹, P. Ahmed ¹, A. C. Sarmah ¹

Affiliations
1 Krishi Vigyan Kendra (A.A.U.), Tinsukia (Assam), IN

Abstract

The present study describes the ITK of pest management practiced by the farmers of Tinsukia districts of Assam. The information was collected on the basis of personal interview to each of the farmer through a questionnaire. A total sample of 200 farmers of twenty different villages was selected randomly from three rural developmental blocks of the district. All in about 30 ITKs were collected and documented. The percentage of farmers practicing different ITKs ranged from 11 to 78.65 per cent. Identification of scientifically sound indigenous practices may serve as the input for valid scientific management for large scale use of insect pest management and in generation of low cost, location specific and appropriate technology.

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R. K. Nath ¹, D. K. Saikia ², P. Ahmed ¹

Affiliations
1 Krishi Vigyan Kendra (A.A.U.), Tinsukia (Assam), IN
2 Department of Entomology, Assam Agricultural University, Jorhat (Assam), IN

Abstract

Field experiment was conducted to investigate the nature of damage caused by sugarcane plassey borer C. tumidicostalis. Generally, there are two phases of infestation of the pests known as primary and secondary infestation. From the investigation, it was observed that the mean number of internodes damaged was more or less similar (1 to 3 internodes) in both the primary and secondary infestation. The mean number of damaged internode was 1.88, 2.06 and 2.42 which was slightly more in the secondary infested canes with 1.78, 2.56 and 2.74 in August, September and October, respectively. However, there was a great variation in the length of spindle damaged by the larvae in primary and secondary infested cane. Similarly, the mean length of internode damaged was more in primary infested canes than secondary infested canes. The mean proportion damaged length caused by the larvae in primary infested cane was 0.46, 0.45 and 0.41 during August, September and October as against 0.18, 0.13 and 0.16 in secondary infested cane, respectively.

Keywords

Plassey Borer, Primary Infestation, Secondary Infestation, Spindle.

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K. H. Begum ¹, R. K. Nath ², P. Ahmed ²

Affiliations
1 District Agriculture Office, Tinsukia (Assam), IN
2 Krishi Vigyan Kendra (A.A.U.), Tinsukia (Assam), IN

Abstract

An experiment was conducted in the Horticultural orchard of Assam Agricultural University, Jorhat from 2014-15 and 2015-16 to develop IPM module against pest and diseases of potato. Ten different IPM modules were followed against the target pest and diseases. Out of these, the IPM module consisting of soil application of MOC followed by high ridge planting and use of yellow sticky trap followed by application of malathion 5 per cent dust and Ridomil MZ-72 @ 0.01% recorded lowest infestation of mole cricket and red ant with 3.42 and 7.71 per cent, respectively with a highest yield of 248.61 q/ha. Likewise, the per cent increase in yield and benefit cost ratio was observed highest (8.54) in the module with low incidence of late blight and leaf roll disease.

Keywords

IPM, Mole Cricket, Red Ant, Malathion, Ridomil MZ-72.

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